Optimization of extraction conditions for the recovery of phenolic compounds and antioxidants from Syrian olive leaves
- Publish Date: 2016-10-01
In this study the effect of extraction conditions, solvent (type, composition), time and temperature, on the amount of phenolic compounds extracted from olive leaves obtained from Safita /Syria was investigated and the radical scavenging activity of these extracts was evaluated. The total phenolic content and the radical scavenging activity of olive leaves extracts were determined by Folin-Ciocalteu reagent and scavenging of DPPH (2,2- diphenyl-1-picrylhydrazyl) radical, respectively. It was found that concentration of solvents (40% ethanol, 40% methanol, 80% acetone, and 6:2: acetone: ethanol: water) gave the highest total phenolic content and possess strong activity against the DPPH radicals (IC50 18.92, 25.01, 33.15, 37.19 μg Gallic acid /ml), respectively.