Microbiological and Analytical Evaluation of Semi-Solid Formulations of Do xycycline Hy clate under Acceler ated Stability Conditions

• Publish Date: 2025-11-11

Research Abstract:

Doxycycline Hyclate (DOX) is a broad-spectrum antibiotic that belongs to the tetracycline family. It has been widely used in the treatment of several inflammatory diseases, and is considered the first-line therapy in the management of moderate to severe cases of acne. In this research, Doxycycline was formulated in four semi-solid formulations (F1and F2 as Gels, F3 and F4 as ointments), then these formulations were subjected to accelerated stability conditions for three months. The formulations were evaluated using microbiological and analytical methods after one and three months. Agar well diffusion method was used as a microbiological method to screen the antibacterial activity of semi-solid formulations against two types of bacteria, Staphylococcus Aureus and Pseudomonas Aeruginosa. HPLC was used as an analytical method for the quantitative and qualitative determination of these formulations.

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