Automated Breast Cancer Detection Using FISH Spectral Linear Unmixing
- تاريخ النشر: 2015-08-06
Fluorescence microscopy plays an important role in the classification of cancerous Tissue. The dramatic increase in multicolor fluorescence microscopy applications witnessed over the past decade is due, in part, to the significant advances in instrument and detector design. A number of advanced microscopy techniques have been applied using multi-color fluorescence labeling, including fluorescence recovery after photo bleaching (FRAP), fluorescence correlation spectroscopy (FCS), fluorescence resonance energy transfer (FRET), fluorescence in situ hybridization (FISH), and fluorescence lifetime imaging (FLIM). Many of these methods benefit significantly from the ability to use specifically targeted fluorescent proteins in live-cell imaging experiments.