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In vitro Evaluation of Helicobacter pylori and Ureases Inhibition Activity of Syrian Honey Samples
(E-pub Ahead of Print)

Author(s): Wissam Zam*, Aziz Khaddour, Hala Suliman.

Journal Name:Current Enzyme Inhibition

Volume 13 , 2017

DOI : 10.2174/1573408013666170929122514


Background: considering that Helicobacter pylori, a bacterium associated with a number of important upper gastrointestinal conditions, not always can be effectively eradicated by the traditional approaches, there is an interest in alternative therapies. Honey has been used as a medicine since ancient times in many countries for its elevated antioxidant activity, as well as expressive antimicrobial activity. Objective: the objective of this study was to investigate the effecting degree of H. pylori with different type of honey samples from Syria. Method: micro-dilution method and agar-well diffusion assay were carried out on H. pylori isolated from gastric biopsy. Inhibition of H. pylori urease was also tested. Results: all honey samples inhibited the growth of H. pylori with a MIC ranging from 30-50%. Both Black seed honey and Coastal Mountain honeys at 50% (v/v%) showed a growth inhibition zone of 16 and 15 mm, respectively; being similar to the growth inhibition zone of Amoxicillin (15 mm) and Levofloxacin (14 mm). 65% of H. pylori urease activity was inhibited using Black seed honey and Coastal Mountain at 50% (v/v%). Conclusion: These results indicate that Black seed and Coastal Mountain honey samples may be appropriate agents to treat H. pylori.

Keywords: Syrian honey samples; Helicobacter pylori; MIC; inhibition zone diameter; urease inhibition